Reading: Seed-sterilization of Rhododendron wardii for micropropagation

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Seed-sterilization of Rhododendron wardii for micropropagation

Authors:

R. M. N. T. Amarasinghe,

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan, CN
About R. M. N. T.

Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China

 

Agriculture Research and Development Centre, Horticulture Research and Development Institute, Department of Agriculture, Sri Lanka
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J-H. Wang,

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan, CN
About J-H.
Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China
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W-X. Xie ,

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan
About W-X.
Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China
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L-C. Peng,

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan, CN
About L-C.
Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China
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S-F. Li,

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan, CN
About S-F.
Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China
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H. Li

Flower Research Institute of Yunnan Academy for Agricultural Sciences, Kunming,Yunnan, CN
About H.
Yunnan Flower Breeding Key Laboratory, Kunming, Yunnan, China
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Abstract

Success in tissue culture of all plant species depends on the removal of exogenous and endogenous contaminating microorganisms. Rhododendron wardii is a parent of many of the best yellow hybrids and it has been identified as a Least Concern spp. An efficient protocol was developed for sterilization of seeds of R. wardii for in vitro cultivation for mass propagation and conservation. Three sterilizing agents, namely, HgCl2 (w/v), NaOCl (v/v) + Tween20 and H2O2 (v/v) at varying their concentration and time of exposure were tested. Treatment with 25% H2O2 for 20 min was the most effective with no contaminated cultures, where the germination percentage was 92.6% (ex vitro germination percentage was 58.61%) and produced 100% healthy plants. Even though the exposure to 15% NaOCl + Tween 20 for 20 min produced 100% contamination-free cultures, the percentage seed germination was 51%. Seeds could be sterilized with 5% NaOCl, for 20 min and 15% for 10 min for maximum germination (64%), but contamination rate was 1:2. Sterilization with HgCl2 did not produce any satisfactory result (maximum germination percentage = 18%).

How to Cite: Amarasinghe, R.M.N.T., Wang, J.-H., Xie, W.-X., Peng, L.-C., Li, S.-F. and Li, H., 2018. Seed-sterilization of Rhododendron wardii for micropropagation. Sri Lanka Journal of Food and Agriculture, 4(1), pp.9–14. DOI: http://doi.org/10.4038/sljfa.v4i1.51
Published on 30 Oct 2018.
Peer Reviewed

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