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Detection of Peste des Petits Ruminants Virus genome in fecal material of goats after an outbreak in Punjab Province of Pakistan: A longitudinal study

Authors:

R. Wasee Ullah ,

Veterinary Research Institute, Zarar Shaheed Road, Lahore, PK
About R. Wasee

Quaid-i-Azam University Islamabad Pakistan

Department of Microbiology, Faculty of Biological Sciences
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A. Bin Zahur,

National Agricultural Research Centre, Islamabad, PK
About A. Bin
Animal Health Research Laboratories, Animal Sciences Institute
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H. Irshad,

National Agricultural Research Centre, Islamabad, PK
About H.
Animal Health Research Laboratories, Animal Sciences Institute
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A. Latif,

National Agricultural Research Centre, Islamabad, PK
About A.
Animal Health Research Laboratories, Animal Sciences Institute
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J. Iqbal Dasti,

Quaid-i-Azam University, Islamabad, PK
About J. Iqbal
Department of Microbiology, Faculty of Biological Sciences

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A. Rashid Malik,

Department of Animal Husbandry, Muzaffarabad, Azad Government of the State of Jammu and Kashmir, PK
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M. Afzal

FAO-UN, Islamabad, (GCP/PAK/127/USA), PK
About M.
Progressive Control of PPR in Pakistan
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Abstract

Peste des Petits Ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants causing high morbidity and mortality (up to 100%) and thus, has serious socioeconomic implications. In Pakistan, estimated losses due to PPR during the year 2012-13 were worth Pakistan Rs. 31.51 billion. Close contact between infected and susceptible animal is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of the disease. Limited information is available about the persistence of PPR virus in goats. Hence, the objective of the study was to investigate the persistence of PPR virus (PPRV) in goats recovered from PPR. A suspected PPR outbreak in sub-urban area of Lahore district of the Punjab Province in Pakistan was investigated. The outbreak was confirmed as PPR after analyzing appropriate samples (nasal/ocular swabs, fecal and tissue samples) collected from the infected animals. A longitudinal study was conducted over a period of 16 weeks to ascertain the persistence of PPRV in the fecal samples of recovered goats (n=96) collected at 4, 8, 12 and 16 weeks after the outbreak. Samples were analyzed using RT-PCR. Of the 96 fecal samples, 46, 37, 29 and 7 goats remained positive for PPRV genome at 4, 8, 12 and 16 weeks after recovery, respectively. The results revealed the detection of PPRV genome in goats at 16 weeks after recovery. Therefore, it can be concluded that PPR disease has a carrier potential and goats recovered from PPR infection may act as source of carrier animals.
How to Cite: Ullah, R.W., Zahur, A.B., Irshad, H., Latif, A., Dasti, J.I., Malik, A.R. and Afzal, M., 2015. Detection of Peste des Petits Ruminants Virus genome in fecal material of goats after an outbreak in Punjab Province of Pakistan: A longitudinal study. Sri Lanka Journal of Food and Agriculture, 1(2), pp.31–40. DOI: http://doi.org/10.4038/sljfa.v1i2.17
Published on 12 Sep 2015.
Peer Reviewed

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