Reading: Formulation of low cost artificial diet for Rrearing diamondback moth (Plutella xylostella L.)

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Research Papers

Formulation of low cost artificial diet for Rrearing diamondback moth (Plutella xylostella L.)

Authors:

M.T.M.D.R. Perera ,

Plant Quarantine Unit, Gannoruwa, Peradeniya, LK
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N. Senanayake,

Department of Plant Science, Faculty of Agriculture, Rajarata University of Sri Lanka, Puliyankulama, LK
About N.
Department of Plant Science, Faculty of Agriculture
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A.G.P.K. Premachandra

Rajarata University of Sri Lanka, Puliyankulama, LK
About A.G.P.K.
Department of Plant Science, Faculty of Agriculture
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Abstract

Use of biological agents for insect pest control is important to prevent degradation of natural resources and for consumers to have insecticide residue-free vegetables. Launching of such programmes to use parasites and parasitoids as biocontrol agents for insect pest control need rearing crop pests for parasitization purposes. Mass rearing of the diamondback moth (DBM; Plutella xylostella L.) in the laboratory using artificial diet is necessary to have a continuous supply of parasitoids for field release. Experiments were conducted in the Plant Quarantine Unit at Gannoruwa in Peradeniya, Sri Lanka using second instar DBM larvae. Nine artificial diets (AD1 to AD9) were formulated for rearing DBM and compared with cabbage (Brassica oleracea L.) leaves as the control. The AD2 to AD9 were formulated by modifying the expensive Biever and Boldt diet (AD1) with low cost material i.e. partial replacement of agar with gelatin, Casein with soya flour, Biever’s yeast with ordinary yeast, raw wheat germ with ‘semolina’, Alphacel with filter papers, aureomycin with tetracycline, and linseed oil with vegetable oil. The results indicated that all the treatments had less survival rate of the DBM than the control. The percentage survival of the DBM on AD7, AD4, AD8 and AD5 diets were 48, 60, 60 and 72, respectively, compared to the control. The length of larvae was significantly higher (p<0.05) in all treatments compared to control and D5 diets. The larval period in AD5 diet (8-12 days), though extended, was comparable to control (8-10 days). However, the mean weight of pupae reared on AD5 diet was significantly higher (p<0.05; 5.2 mg) than those of other diets. The number of males emerging from AD2, AD4, AD8 and AD9 diets were higher than others whereas control and AD5 gave similar results (p>0.05). Eggs laid per female were low in DBM reared in all artificial diets. The length of life cycle was low in AD5 (15-19 days) comparable to other diets. Overall results indicated that the AD4, AD5 and AD8 were better than other diets while AD5 was comparable to the control suggesting that this diet can be used for mass rearing of DBM in the laboratory.
How to Cite: Perera, M.T.M.D.R., Senanayake, N. & Premachandra, A.G.P.K., (2015). Formulation of low cost artificial diet for Rrearing diamondback moth (Plutella xylostella L.). Sri Lanka Journal of Food and Agriculture. 1(1), pp.25–33. DOI: http://doi.org/10.4038/sljfa.v1i1.4
Published on 01 Mar 2015.
Peer Reviewed

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