Reading: Effect of fenugreek leaf extract (Trigonella foenum-graecum L.) on in vitro methanogenesis a...

Download

A- A+
dyslexia friendly

Research Papers

Effect of fenugreek leaf extract (Trigonella foenum-graecum L.) on in vitro methanogenesis and fermentation of wheat straw-based diet (Triticum aestivum L.) fed to buffaloes

Authors:

A. Dey ,

Central Institute for Research on Buffaloes, Hisar, IN
About A.
Division of Animal Nutrition and Feed Technology, ICAR
X close

S.S. Paul,

Central Institute for Research on Buffaloes, Hisar, IN
About S.S.
Division of Animal Nutrition and Feed Technology, ICAR
X close

P. Pandey,

IN
About P.
Division of Animal Nutrition and Feed Technology, ICAR
X close

B.S. Punia,

Central Institute for Research on Buffaloes, Hisar, IN
About B.S.
Division of Animal Nutrition and Feed Technology, ICAR
X close

S.S. Dahiya,

Central Institute for Research on Buffaloes, Hisar, IN
About S.S.
Division of Animal Nutrition and Feed Technology, ICAR
X close

P.C. Lailer,

Central Institute for Research on Buffaloes, Hisar, IN
About P.C.
Division of Animal Nutrition and Feed Technology, ICAR
X close

N. Saxena

Central Institute for Research on Buffaloes, Hisar, IN
About N.
Division of Animal Nutrition and Feed Technology, ICAR
X close

Abstract

The secondary metabolites present in some plants have the potential in modifying rumen fermentation due to their antimicrobial activity. The present study aimed investigating the effect of dosing extracts of fenugreek (Trigonella foenum-graecum L.) leaves on in vitro methanogenesis and fermentation of feed in buffalo (Bubalus bubalis Linnaeus, 1758). The leaves of fenugreek were dried at 45-50 °C and ground to pass through 1 mm sieve. Extracts (100 ml) were prepared with 10 g powdered sample using three solvents namely, ethanol, methanol and water, and stored at 4 °C for subsequent use. The extracts were tested at three levels (0, 1.0 and 2.0 ml). A 60:40 mixture (200 ± 5 mg) of wheat (Triticum aestivum L.) straw and concentrate feed was used as substrate and incubated with 30 ml buffered rumen fluid in 100 ml calibrated glass syringes at 39 °C for 24 hrs following the standard IVGP protocol. Gas production was recorded by displacement of piston in the syringe. Methane in the gas phase and volatile fatty acids (VFA) in the fermentation medium were estimated by GLC. The total gas and methane production was reduced significantly (p<0.001) with the increase in microbial biomass production by inclusion of methanol extract of fenugreek leaves. Acetate production was reduced with increase of propionate, which reduced the acetate to propionate ratio (p<0.05). The truly degradable dry matter content was increased by the addition of methanol extract. Results of this study suggested the potential use of methanol extract of fenugreek leaves as an anti-methanogenic feed additive to improve rumen fermentation in buffaloes.

How to Cite: Dey, A. et al., (2015). Effect of fenugreek leaf extract (Trigonella foenum-graecum L.) on in vitro methanogenesis and fermentation of wheat straw-based diet (Triticum aestivum L.) fed to buffaloes. Sri Lanka Journal of Food and Agriculture. 1(1), pp.9–13. DOI: http://doi.org/10.4038/sljfa.v1i1.2
Published on 01 Mar 2015.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus