Reading: Effect of L-carnitine enrichment on the rotifer and influences on clownfish larvae

Download

A- A+
Alt. Display

Research Papers

Effect of L-carnitine enrichment on the rotifer and influences on clownfish larvae

Authors:

S. Ghosh ,

Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu and "Sasya Shyamala" Krishi Vigyan Kendra, Indian Council of Agricultural Research (ICAR), School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, Kolkata, IN
X close

T.T. Ajith Kumar,

Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, IN
X close

N.C. Sahu

"Sasya Shyamala" Krishi Vigyan Kendra, Indian Council of Agricultural Research (ICAR), School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, Kolkata, IN
X close

Abstract

Enrichment has shown considerable influence on the population growth, reproduction and individual growth of rotifer (Brachionus plicatilis Müller, 1786) and its utility on the clownfish larvae (Amphiprion ocellaris Cuvier 1830). Using the culture medium of microalgae (Nannochloropsis salina D.J. Hibberd 1981) as the control, rotifers were enriched by dissolving in S-presso (for short term enrichment) or L-carnitine supplements. The study was conducted in three replicates for five and 10 days of batch culture. On day-4, the population density of rotifers exposed to 1, 10 and 100 mg l -1 L-carnitine was significantly increased (p<0.001) by 43, 39 and 54%, respectively, compared to the control. The population density in these three treatments also increased on day-2, day-3 and day-5 compared to the 1000 mg L-1 treatment (p<0.05) and control (p<0.05). The body length (p<0.001) and width (p<0.05) were significantly reduced in the 1000 mgL-1 treatment compared to the control. The mean values of total unsaturated fatty acids (PUFA's, HUFA's and n-3 fatty acids) and the ratio of n-3/n-6, DHA/EPA, EPA/AA were significantly higher (p<0.05) in the rotifers enriched with L-carnitine compared to S-presso and algae. Larvae fed using rotifer enriched with L-carnitine gained the maximum growth (65.7±1 mg), followed by those enriched with S-presso (42.2±0 mg). In L-carnitine treatment, metamorphosis took only 9 days, followed by the control. In L-carnitine and S-presso units, the clownfish larvae took only three days for the first pigmentation compared to four days in N. salina (control) and 10 days with S-presso. Rotifers enriched with L-carnitine showed the highest survival rate (70 %) of the clownfish followed by the control (68%) and S-presso (52%). The results suggested that L-carnitine could be a positive factor to enhance the rotifer production and also its utilization in clownfish larval culture.

How to Cite: Ghosh, S., Kumar, T.T.A. and Sahu, N.C., 2015. Effect of L-carnitine enrichment on the rotifer and influences on clownfish larvae. Sri Lanka Journal of Food and Agriculture, 1(1), pp.71–84. DOI: http://doi.org/10.4038/sljfa.v1i1.10
Published on 01 Mar 2015.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus